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Full Record Details
Persistent URL
http://purl.org/net/epubs/work/49081941
Record Status
Checked
Record Id
49081941
Title
Covalently bonded hopanoid-Lipid A from Bradyrhizobium: The role of unusual molecular structure and calcium ions in regulating the lipid bilayers organization
Contributors
G Vitiello
,
R Oliva
,
L Petraccone
,
PD Vecchio
,
RK Heenan (STFC Rutherford Appleton Lab.)
,
A Molinaro
,
A Silipo
,
G D'Errico
,
L Paduano
Abstract
Lipopolysaccharides (LPS) are complex amphiphilic macromolecules forming the external leaflet of the outer membrane of Gram-negative bacteria. The LPS glycolipid portion, named Lipid A, is characterized by a disaccharide backbone carrying multiple acyl chains. Some Lipid A bear very-long-chain-fattyacids (VLCFA), biosynthesized to span the entire lipid membrane profile. The synbiontic Bradyrhizobium BTAi1 strain carries an unique Lipid A specie, named HoLA, in which VLCFA terminus is covalently-bonded to hopanoid, a triterpenoid displaying structural similarity with eukaryotic sterols. Here, we investigate the role of HoLA in regulating self-assembly, microstructure and thermotropicity of lipid membranes composed by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-10-rac-glycerol. DLS and SANS indicated the formation of multilamellar aggregates whose size increases when the hopanoid is present in the bilayer. EPR and DSC showed that HoLA induced a more rigid and ordered organization among the lipids in the bilayer, evocative of a mechanical strengthening. Notably, the presence of calcium ions promote an increase of the bilayer thickness and vesicles size, leading to low curvature aggregates. These results highlight the key role of the hopanoid covalently-linked to VLCFA in defining the physico-chemical properties of bacterial envelope, thus offering a robust scientific basis for the interpretation of the biological activity of the considered strain.
Organisation
ISIS
,
ISIS-LOQ
,
STFC
Keywords
SasView
,
RB1710351
Funding Information
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Language
English (EN)
Type
Details
URI(s)
Local file(s)
Year
Journal Article
J Coll Int Sci
594 (2021): 891-901.
doi:10.1016/j.jcis.2021.03.072
2021
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