@ARTICLE{TreeBASE2Ref29468,
author = {Mark Z. Nemeth and Alexandra Pintye and ?ron N. Horv?th and P?l V?gi and G?bor M. Kov?cs and Markus Gorfer and Levente Kiss},
title = {Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction},
year = {2019},
keywords = {tritrophic interactions},
doi = {10.1094/PHYTO-01-19-0013-R},
url = {http://apsjournals.apsnet.org/doi/10.1094/PHYTO-01-19-0013-R},
pmid = {30900938},
journal = {Phytopathology},
volume = {109},
number = {8},
pages = {1404--1416},
abstract = {Powdery mildews, ubiquitous obligate biotrophic plant pathogens, are often attacked in the field by mycoparasitic fungi belonging to the genus Ampelomyces. Some Ampelomyces strains are commercialized biocontrol agents of crop pathogenic powdery mildews. Using Agrobacterium tumefaciens-mediated transformation (ATMT), we produced stable Ampelomyces transformants that constitutively expressed green fluorescent protein (GFP) to (i) improve the visualization of the mildew? Ampelomyces interaction and (ii) decipher the environmental fate of Ampelomyces fungi before and after acting as a mycoparasite. Detection of Ampelomyces structures, and especially hyphae, was greatly enhanced when diverse powdery mildew, leaf, and soil samples containing GFP transformants were examined with fluorescence microscopy compared with brightfield and differential interference contrast optics. We showed for the first time, to our knowledge, that Ampelomyces strains can persist up to 21 days on mildew-free host plant surfaces, where they can attack powdery mildew structures as soon as these appear after this period. As saprobes in decomposing, powdery mildew-infected leaves on the ground and also in autoclaved soil, Ampelomyces strains developed new hyphae but did not sporulate. These results indicate that Ampelomyces strains occupy a niche in the phyllosphere where they act primarily as mycoparasites of powdery mildews. Our work has established a framework for a molecular genetic toolbox for the genus Ampelomyces using ATMT.}
}
Matrix 18594 of Study 23290
Citation title:
"Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction".
Study name:
"Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction".
This study is part of submission 23290
(Status: Published).
Matrices
Title: MSA OG7d
Rows
Taxon Label |
Row Segments |
Characters 1?–30 |
Arabidopsis thaliana ANAC044 At3g01600 1 |
(none)
|
RAWIVGRGIAAKVKNASLSSALQIQDCG-- |
Arabidopsis thaliana ANAC085 At5g14490 1 |
(none)
|
RTWIVGPWISRNVKNASLSSALQIKDCG-- |
Musa acuminata GSMUA Achr9T01880 001 |
(none)
|
RTWLIGRGIARKVKNANCFDNDQIKDLGVG |
Musa acuminata GSMUA AchrUn randomT17050 001 |
(none)
|
RTWLIGRGIARKVKNATFSDN-QIKDIGVE |
Oryza sativa Os02g38130 1 |
(none)
|
RSWLIGRGVAKKIRNAPHCSSRPISELGAE |
Oryza sativa Os04g40140 1 |
(none)
|
RPWIIGKVIATKIRNATQLLSCKLGELVPE |
Vitis vinifera VvNAC59 GSVIVT01032388001 |
(none)
|
RTWVVSRGIARKVKNATQSFASQIKDCG-- |
Columns
None of the columns has a description.