@ARTICLE{TreeBASE2Ref15674,
author = {L. D. Guo and Kevin D Hyde and Edward C. Y. Liew},
title = {Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences.},
year = {2001},
keywords = { molecular identi?cation; molecular phylogenetics; environmental DNA; mycelia sterilia; ribosomal RNA gene},
doi = {10.1006/mpev.2001.0942},
url = {},
pmid = {},
journal = {Molecular Phylogenetics and Evolution},
volume = {20},
number = {1},
pages = {1--13},
abstract = {The 5.8S gene and flanking internal transcribed spacers (ITS1 and ITS2) of the rDNA were amplified from total DNA extracted from frond tissues of Livistona chinensis using universal and fungal specific primers. These amplified fragments were cloned and sequenced. Phylogenetic analysis based on the 5.8S gene sequences indicated that the six clone sequences obtained were of different origins. Five sequences, P1-9, P2-6, P4-4, P4-5, and P4-7, belonged to the fungi and one sequence, P3-2, belonged to the plants. P1-9 was inferred to belong to the Basidiomycota based on the phylogenetic analysis of the 5.8S gene sequences but could not be identified to lower taxonomic levels. Further identification of the other four fungal clones to lower taxonomic levels were conducted based on phylogenetic analysis and sequence comparison of both the conserved 5.8S gene and the variable ITS regions. The origin of P2-6 was identified to be Glomerella and its anamorph Colletotrichum; the origins of P4-5 and P4-7 were Mycosphaerella and its anamorph Cladosporium; while P4-4 was from the Herpotrichiellaceae. The direct approach to detecting and taxonomic placement of endophytic fungi within host tissue without the need for conventional in vitro culturing is discussed.}
}
Matrix 2269 of Study 683
Citation title:
"Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences.".
This study was previously identified under the legacy study ID S522
(Status: Published).
Matrices
Title: 5.8S P1-9
Description: Legacy TreeBASE Matrix ID = M762
Rows
|
Taxon Label |
Row Segments |
Characters 1?–30 |
| Glomerella graminicola |
(none)
|
AAACTTTTAACAACGGATCTCTTGGTTCTG |
| Hypocrea rufa |
(none)
|
AAACTTTCAACAACGGATCTCTTGGTTCTG |
| Xylaria hypoxylon |
(none)
|
AAACTTTCAACAACGGATCTCTTGGTTCTG |
| Pleospora herbarum |
(none)
|
CAACTTTCAACAACGGATCTCTTGGTTCTG |
| Saccharomyces cerevisiae |
(none)
|
AAACTTTCAACAACGGATCTCTTGGTTCTC |
| Glomus mosseae |
(none)
|
TCACTTTCAACAACGGATCTCTTGGCTCTC |
| Gigaspora albida |
(none)
|
AAACTTTCAACAATGGATCTCTTGGCTCTC |
| Ceratobasidium oryzae-sativae |
(none)
|
AAAGTTTCAACAACGGATCTCTTGGCTCTC |
| Thanatephorus cucumeris |
(none)
|
TAAGTTTCAACAACGGATCTCTTGGCTCTC |
| Phaffia rhodozyma |
(none)
|
AAACTTTCAACAACGGATCTCTTGGTTCTC |
| Xanthophyllomyces dendrorhous |
(none)
|
AAACTTTCAACAACGGATCTCTTGGTTCTC |
| Cystofilobasidium capitatum |
(none)
|
TAACTTTCAACAACGGATCTCTTGGTTCTC |
| Sorosporium saponariae |
(none)
|
CAACTTTCGGCAACGGATCTCTAGGTTCTC |
| Ustilago vaillantii |
(none)
|
CAACTTTCGGCAATGGACCTCTTGGTTCTC |
| Sporisorium sorghi |
(none)
|
CAACTTTTGGCAACGGATCTCTTGGTTCTC |
| P1-9 |
(none)
|
TAACTTTTGACAACGGATCTCTTGGTTCTC |
Columns
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Citation
Taxa
Matrices
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