@ARTICLE{TreeBASE2Ref22866,
author = {Yanfang Yang and Hainan Zhao and Roerto Barrero and Baohong Zhang and Guiling Sun and Iain Wilson and Fuliang Xie and Kevin D Walker and Joshua Parks and Robet Bruce and Guangwu Guo and Li Chen and Yong Zhang and Xin Huang and Tang Qi and Hongwei Liu and Matthew Bellgard and Angela Hoffman and Jinsheng Lai and Deyou Qiu},
title = {Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431},
year = {2014},
keywords = {Penicillium aurantiogriseum NRRL 62431, Paclitaxel, Taxol?, Endophytic fungi, Genome sequence, Horizontal gene transfer},
doi = {10.1186/1471-2164-15-69},
url = {http://www.biomedcentral.com/1471-2164/15/69/abstract},
pmid = {24460898},
journal = {BMC Genomics},
volume = {15},
number = {},
pages = {69},
abstract = {Background: Paclitaxel (Taxol?) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer.
Results: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely.
Conclusions: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will
facilitate metabolic engineering for the industrial production of paclitaxel from fungi.}
}
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Citation title: "Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431".
Study name: "Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431".
This study is part of submission 15183
(Status: In Progress).
Citation
Yang Y., Zhao H., Barrero R., Zhang B., Sun G., Wilson I., Xie F., Walker K.D., Parks J., Bruce R., Guo G., Chen L., Zhang Y., Huang X., Qi T., Liu H., Bellgard M., Hoffman A., Lai J., & Qiu D. 2014. Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431. BMC Genomics, 15: 69.
Authors
Yang Y.
Zhao H.
Barrero R.
Zhang B.
Sun G.
Wilson I.
Xie F.
Walker K.D.
Parks J.
Bruce R.
Guo G.
Chen L.
Zhang Y.
Huang X.
Qi T.
Liu H.
Bellgard M.
Hoffman A.
Lai J.
Qiu D.
Abstract
Background: Paclitaxel (Taxol?) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer.
Results: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely.
Conclusions: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will
facilitate metabolic engineering for the industrial production of paclitaxel from fungi.
@ARTICLE{TreeBASE2Ref22866,
author = {Yanfang Yang and Hainan Zhao and Roerto Barrero and Baohong Zhang and Guiling Sun and Iain Wilson and Fuliang Xie and Kevin D Walker and Joshua Parks and Robet Bruce and Guangwu Guo and Li Chen and Yong Zhang and Xin Huang and Tang Qi and Hongwei Liu and Matthew Bellgard and Angela Hoffman and Jinsheng Lai and Deyou Qiu},
title = {Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431},
year = {2014},
keywords = {Penicillium aurantiogriseum NRRL 62431, Paclitaxel, Taxol?, Endophytic fungi, Genome sequence, Horizontal gene transfer},
doi = {10.1186/1471-2164-15-69},
url = {http://www.biomedcentral.com/1471-2164/15/69/abstract},
pmid = {24460898},
journal = {BMC Genomics},
volume = {15},
number = {},
pages = {69},
abstract = {Background: Paclitaxel (Taxol?) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer.
Results: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely.
Conclusions: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will
facilitate metabolic engineering for the industrial production of paclitaxel from fungi.}
}
TY - JOUR
ID - 22866
AU - Yang,Yanfang
AU - Zhao,Hainan
AU - Barrero,Roerto
AU - Zhang,Baohong
AU - Sun,Guiling
AU - Wilson,Iain
AU - Xie,Fuliang
AU - Walker,Kevin D
AU - Parks,Joshua
AU - Bruce,Robet
AU - Guo,Guangwu
AU - Chen,Li
AU - Zhang,Yong
AU - Huang,Xin
AU - Qi,Tang
AU - Liu,Hongwei
AU - Bellgard,Matthew
AU - Hoffman,Angela
AU - Lai,Jinsheng
AU - Qiu,Deyou
T1 - Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
PY - 2014
KW - Penicillium aurantiogriseum NRRL 62431
KW - Paclitaxel
KW - Taxol?
KW - Endophytic fungi
KW - Genome sequence
KW - Horizontal gene transfer
UR - http://www.biomedcentral.com/1471-2164/15/69/abstract
N2 - Background: Paclitaxel (Taxol?) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer.
Results: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely.
Conclusions: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will
facilitate metabolic engineering for the industrial production of paclitaxel from fungi.
L3 - 10.1186/1471-2164-15-69
JF - BMC Genomics
VL - 15
IS -
ER -